The effect of Lactobacillus acidophilus supplementation on serum lipid profile and Renal Amyloid protein and TNF-alpha levels in rats fed a high-fat diet

Objective: In this study, it was aimed to investigate the effect of Lactobacillus acidophilus supplementation on serum lipid profile and kidney total beta amyloid protein (TBP) and TNF-alpha levels in rats exposed to an experimental high-fat diet. Material and Method: In the study, 4 groups were formed using 24 male Sprague-Dawley rats. Control given standard rat chow (SD), Group1; high-fat diet (YYD) fed, Group 2; Consuming SD and administered Lactobacillus acidophilus probiotic, Group 3; It was grouped as Group 4, which was fed YYD and applied Lactobacillus acidophilus probiotic. Body weights were determined weekly during the 12-week trial period. At the end of the experiment, TBP and TNF-α levels were measured in the serum and kidney tissue of the rats by ELISA method. Total cholesterol (TC), triglyceride (TG), HDL, LDL, urea and creatinine levels and paraoxanase, amylase and lipase activities in serum were determined by spectrophotometric method in the analyzer device. Results: At the end of the experiment, when the control (Group 1) group and Group 2 were compared, it was determined that the weight gain was the highest in Group 2, the amount of serum TG, TK, LDL and urea, and the levels of TNF-α and TBAP in the serum and kidney tissue were significantly higher (P<0.05)). Serum HDL, PON and amylase levels were found to be significantly low (P>0.05). When the groups treated with Lactobacillus acidophilus probiotic (Group 3, 4) were compared with Group 2, it was determined that there was a decrease in TG, TK, LDL, urea levels, and the amount of TNF-α and TBAP in the serum and kidney tissue (P>0.05). It was observed that HDL, PON and amylase levels increased and approached the control group (P<0.05). Conclusion: As a result of the study, it was observed that probiotic supplementation had a positive effect on TG, TK, HDL, LDL, urea, PON and amylase levels in serum and TNF-α and TBAP levels in serum and kidney tissue in rats created obese with a high-fat diet.