Determination of zofenopril and hydrochlorothiazide by capillary electrophoresis and high performance liquid chromatography in pharmaceutical preparations

In this study, a capillary electrophoresis (KE) method was developed for the determination of zofenopril (ZOF) and hydrochlorothiazide (HCT) from pharmaceutical preparations and for comparison high performance liquid chromatography (YPSK) method is used. Telmisartan (TEL) was used as internal standard (IS) and the detection was achieved by photo diode array (DAD) detector in the methods. In the CE method, the separation was performed by bubble cell capillary with an inner diameter of 75 µm and an effective length of 40 cm. Optimum conditions were obtained by applying 10 mM borate buffer containing 15% methanol (pH 9,00) and 25 kV potential. Under these conditions, the migration times of ZOF, HCT and IS were observed as 4,67 min, 3,38 min and 4,25 min. In the YPSK method, for separation C8 column (3,0×150,0 mm, 3,5 µm i.d.) as mobile phase; 10% acetonitrile containing 1% phosphoric acid (H3PO4) and acetonitrile containing 1% H3PO4 solutions were used in gradient system at a flow rate of 1 mL/min. In this method, the retention times of ZOF, HCT and IS were observed as 9,05 min, 1,89 min and 6,53 min, respectively. Validation of the developed methods were examined by linearity, precision, accuracy, selectivity, sensitivity and stability parameters. The limit of quantification (LOQ) values of ZOF and HCT were 8,28×10-8 M and 6,31×10-8 M in CE and 6,93×10-8 M ve 4,40×10-8 M in YPSK, respectively. Both of the developed methods were successfully applied for the determination of ZOF and HCT from tablets. Keywords: Zofenopril, Hydrochlorothiazide, Capillary electrophoresis, High performance liquid chromatography, Determination