Micropropagation of Adana and around naturally grown carob (Ceratonia siliqua L.) genotypes with the classic and new generation tissue culture techniques and determination of genetic stability

In this study, micropropagation and rooting studies were carried out in comparative Plantform bioreactor system and solid media with three different carob genotypes which are growing naturally in the Mediterranean Region. First of all, micropropagation of carob genotyps were evaluated in solid MS, ½ MS and WPM media supplemented with BA (0, 0.5, 1.0, 1.5, 2.0 mg/L) and GA3 (0.1, 0.5 mg/L) for micropropagation, in MS, ½ MS and WPM media supplemented with IBA (0, 1.0, 2.0 mg/L) and NAA (0, 1.0, 2.0 mg/L) for rooting. Based on the solid media, the best results in all three genotypes were obtained from MS and ½ MS medium containing 0.5 and 2 mg/L BA+0.5 mg/L GA3 plant growth regulatory concentrations for micropropagation. Micropropagation studies were carried out in the Plantform system with the best-defined media content. As a result of studies, Plantform system showed better plant growth, multiplication coefficient and plant quality than solid culture system in all three genotypes propagation medium. Genetic stability of plants grown in solid culture and Plantform systems was tested by SSR markers.