Determination of the interaction between aluminium and some organic acids by spectrometric and chromotographic methods

The interest to aluminium which is one of the most abundant element, increases with the knowledge of its harms to biologic and natural systems with its increase of usage recently. Aluminium is an element that can cause norological diseases such as Alzheimer, Parkinson with its harm on human metabolism. Bearing this in mind, some spectrometric determination techniques with some reagents are applied to determine the uptake of aluminium from the solution or to examine this uptake in the presence of different organic acids environment. Moreover, it is possible to clarify its chemical forms with chromotographic determination. Most part of the study was holded in Royal Liverpool University Hospital’s Clinical Biochemistry Laboratories for the project that was supported by British Council and some of it at Czech University, Agricultural Faculty, Soil Science Department for clarifying this effects of aluminium. In this study, tiron and pyrocathecol violet were used to determine aluminium spectrometrically. Additionally, the effects of some compounds such as sitric acid, maltol, lactate, acetate, acethylacetonate were examined for the determination of aluminium in the presence of these reagents. Also behaviour of (-)epicathecin, (-)epigallocatechin were studied for tiron. Some synthetic solutions were examined to separate the chemical forms of aluminium at pH 2,5 and it was tried to determine the forms at this pH by chromotographic determination. Additionally, the aluminium content in some daily used fruit juices and tea samples were analysed with GFAAS and ICP-OES methods. Keywords: Aluminium, spectrometric determination, tiron, pyrocathecol violet, chemical forms of aluminium. xii