Identification of callase component ß-1,3- glucanase gene in Arabidopsis thaliana

In this study, it is aimed to characterize gene or genes encoding the ß-1,3-glucanase components of callase enzyme that has not been identified before, in Arabidopsis thaliana as a modal organism.In this study, 50 genes belongs to ß-1,3-glucanase family 17 were screened by appliying various bioinformatic filters and microarray databases were revealed the expression profiles of these genes. Six candidate genes encoding ß-1,3-glucanase component of callase were determined. Total RNA was isolated from A. thaliana anthers at tetrad stage when callase enzyme is active. The candidate genes profiles in anthers were determined by RT-PCR using gene specific primers. The RT-PCR products obtained with these primers were 767, 609, 890, 840, 557 and 672 bp in lengths. Also the candidate genes expression profiles in somatic tissues were revealed to compare with anthers. PCR products separated in %1 agarose gel electrophoresis were stained with Ethidium Bromide and recorded on U.V. table. We observed that while genes were preferentially expressed in somatic tissues, in anthers during callose degradation At4g14080, At3g23770 and At3g55780 genes were expressed. These results indicate that At4g14080, At3g23770 and At3g55780 genes encoding ß-1,3-glucanase could be concluded to be part of the callase enzyme complex in Arabidopsis.