Heredity of resistance against common bunt in some bread wheat varieties

Wheat is most important basic food source in all around the world. Therefore, it has most wide production and prevalent usage area in agricultural production. Together with growing of the world population, the studies based on wheat focus on more efficiency and increasing of quality. In this study, the predetermined 12 wheat varieties (Alpu 01, Altay 2000, İkizce, Kıraç 66, Sultan 95, Soyer 02, Karahan 99, Nacibey, Yayla 305, Müfitbey, PI178383 and M732154) for resistance were used and resistance sources for using in study were composed with hybridization works for the purpose of understanding of resistance characteristics. For clearly showing of resistance situation, some varieties in differential set of wheat lines against common bunt were also used in the hybridization stage. The disease-reads at F2 grade of combinations obtained with hybridization works were made in field. Heredity forms in point of resistance against common bunt disease were investigated by X2 (chi square) test analysis method in data of the obtained disease reactions. DNA isolation from leaf samples of twelve wheat varieties and 64 combinations obtained from hybrids of these varieties was performed by classic CTABmethod. Isolated DNA samples were amplified by PCR using 20 different ISSR primers. Band analysis of PCR results was carried by Phoretix1DPro software and Jaccard Similarity Matrix was obtained UPGMA method using binary data. Phylogenetic tree that shows genetic similarity and distance among wheat varieties was drawn. Pedigrees of varieties were also considered during evaluation of dendrogram and present of a balanced distribution among hybrids having a common ancestor was observed. Finally, it was seen that in fight against common bunt disease anddevelopment of resistant wheat varieties using molecular methods is more fast and efficient.