Investigation of some sulfonamide compounds In vitro effects on polifenol oxidase enzyme

In this study; Polyphenol oxidase enzyme (PPO) was purified from the fruit of bananas (Musa cavendishii), using the affinity gel with the chemical structure of Sepharose 4B-L-tyrosine-p-amino benzoic acid.In the study, imported banana fruits, used as enzyme source, were obtained from the grocery stores and markets in Siirtprovince.Spectrophotometric experimental studies were carried out by determining the absorbance values at 0 and 1st min at 420 nm.The in vitro effects of 12 different original synthesis sulfonamide derived compounds, starting with DT-F-S1 code and ending with DT-F-S12 code, against PPO enzyme were investigated experimentally.% Activity- [Inhibitor Concentration] graphs were generated by experimental datas.The regression analysis of these graphs was performed to determinethe inhibition constants, IC50 values, for each original sulfonamide derivatives.The other inhibition constant, Ki, was calculated mathematically with the help of the Cheng&Prusoff equation.In the sulfonamide group, DT-F-S8 substance was found to be the best inhibition effect with IC50 value of 39,375 μM.