Effect of 3',4'-Dihydroxyflavonol on cortex oxidant/antioxidant system and transmitter levels in brain ischemia-reperfusion

Survivors of global cerebral ischemia (GCI) are at extreme risk of neurological disorders, including cerebral infarction may lead to neuronal loss, may cause brain damage. In the present study, the effect of 1-week 3',4'-Dihydroxyflavonol (DiOHF) supplementation on the oxidant-antioxidant system and transmitter levels in the cerebral cortex tissue after brain-ischemia reperfusion in rats was investigated. It was performed on male Wistar-albino rats. A total of 28 rats were used in the study and the groups were formed as follows. Control Group (n=6): No anesthesia or surgical procedure was performed on the animals in this group. Sham Group (n=6): After general anesthesia was induced in the animals in this group, the carotid artery regions were opened and closed. After the application, vehicle application was performed for 1 week (1 ml DiOHF vehicle). Ischemia-Reperfusion (I/R) Group (n=8): After the carotid arteries were isolated in the rats under general anesthesia, they were ligated for 30 minutes and ischemia was performed followed by reperfusion. Following reperfusion, 1 ml of DiOHF vehicle was supplemented for 1 week. Ischemia-Reperfusion Group + DiOHF Supplemented (n=8): After the carotid arteries were isolated in rats under general anesthesia, they were ligated for 30 minutes and ischemia was performed followed by reperfusion, and then DiOHF supplementation for 1 week was performed. Results of the study indicate that one-week administration of DiOHF following experimental cerebral ischemia-reperfusion (İ/R) in rats led to an increase in MDA levels that a marker of oxidative activity, in the cerebral cortex tissue, while significantly suppressing antioxidant markers such as GPx, SOD, CAT, and TRx 2. Cerebral I/R also resulted in elevated levels of the neurotransmitters glutamate and GABA.However, one-week post-ischemic reperfusion treatment with DiOHF ameliorated these changes in the frontal cortex tissue. Overall, the findings demonstrate that DiOHF administration for one week after experimental cerebral ischemia-reperfusion in rats exerts beneficial effects on the oxidant/antioxidant system and neurotransmitter levels in cerebral cortex tissue. Keywords: CAT; cerebral cortex; GABA; Glutamate; GPx; MDA; SOD; TRx 2