Effect of 1-week 3',4'-Dihydroxyflavonol supplementation on antioxidant system and BDNF, transmitter levels in cerebellum in cerebral ischemia-reperfusion

Cerebral ischemia-reperfusion (I/R) occurs when blood flow is restored after a temporary interruption and can lead to deterioration in brain functions depending on the time passed. The aim of this study was to determine the effect of cerebral I/R and 1-week 3',4'-Dihydroxyflavonol treatment on the antioxidant/antioxidant system and glutamate, GABA, and BDNF levels in cerebellum tissue in male rats. Experimental groups consisting of 28 Wistar-albino type male rats were formed as follows: 1-Control Group (n=6): No anesthesia or surgical procedure was applied to the animals in this group. 2-Sham Group (n=6): After general anesthesia was induced in the animals in this group, the carotid artery regions were opened and closed. After the application, vehicle application was performed for 1 week (1 ml DiOHF solvent). 3-Ischemia-Reperfusion Group (n=8): After general anesthesia, carotid arteries were isolated in rats and ligated for 30 minutes, ischemia was performed and then reperfusion was performed. 4-Ischemia-Reperfusion + DiOHF Group (n=8): After general anesthesia, carotid arteries were ligated for 30 minutes and ischemia was performed in rats, then reperfusion was performed and then DiOHF supplementation was performed for 1 week. After one week of treatment, the animals were sacrificed under general anesthesia and cerebellum tissues were taken. MDA, GPx, catalase, SOD, thioredoxin, glutamate, GABA and BDNF levels were evaluated in the relevant tissue. I/R increased MDA, glutamate and GABA levels, while GPx, catalase, SOD, thioredoxin and BDNF levels were decreased. However, 1 week of 3',4'-Dihydroxyflavonol treatment corrected the changes that occurred with I/R. The study results show that 1 week of 3',4'-Dihydroxyflavonol treatment after I/R has a positive effect on changes that occur in focal brain I/R. Keywords: Ischemia/Reperfusion; 3',4'-Dihydroxyflavonol treatment; MDA, GPx, catalase, SOD, thioredoxin, glutamate, GABA and BDNF