Grafting of acrylic acid fibrous brushes on crosslinked chitosan surfaces: Application in cytochrom C adsorption and determnation of thermodynamic and kinetic parameters

In this study, chitosan membranes gained cation exchanger property with grafting acrylic acid monomer to chitosan membranes. Obtained chitosan-g-pAA was prepared in two phases to be used on adsorption of alkaline cythcrome c from aqueous solution. Firstly, the chitosan membranes was crosslinked with epichlorohydrin. Secondly, the graft copolymerization of acrylic acid monomer which carries anion exchanger groups provided on crosslinked chitosan, by formation of polyacrylic acid fibrils on the surface of chitosan-g-p(AA) membranes, a supporting material with a good mechanic tolerance to biologic and chemical decomposition had been prepared. Optimization and characterization processes of membranes, prepared by modifying, were carried out. Chitosan membranes had been confirmed with FT-IR spectroscopy and thermal determination identified with DSC.Effects of parameters like pH, temperature, ionic intensity on adsorption quality have been investigated during the process of adsorption with chitosan-g-pAA membranes. Maximum experimentaland theoretical adsorption capacities of membranes were identified as 76,21 and 80,39 mg/ml respectively. As a result of Kd value ,obtainedfrom Langmuir adsorption isotherm model, it had been observed that protein had high affinity against sorbent. Greatness of Freundlich model parameters showed that ion exchanging membrane?s taking of cythchrome c with a high adsorption capacity was easy. High correlation coefficients and compatible results of maximum adsorption capacities showed that adsorption is compatible with quadratic kinetic model. Positive ? H and negative ? G values identified for the chitosan-g-pAA membrane, in ion exchanging chromatography area showed that the adsorption is endothermic and spontaneous respectively.