Micropropagation of Cyclamen persicum species using new generation bioreactor system

Species belonging to the Cyclamen genus, which are becoming increasingly important in the ornamental plant industry, are used as indoor and outdoor ornamental plants. Nowadays, cyclamen is widely preferred, especially as a potted ornamental plant, and is among the significant plant species traded both in our country and internationally. Therefore, breeding and micropropagation studies aimed at improving cyclamen species are of great importance. In this thesis study, to induce callus and embryo formation through the somatic embryogenesis method in Cyclamen persicum, which naturally grows in Türkiye, in vitro solid culture and the new-generation temporary immersion system, Plantform bioreactor system, were used, and their plant conversion efficiencies were comparatively examined. As a preliminary study, leaf and petiole explants were cultured in a medium containing ½ MS along with 2 mg/L 2,4-D and 0.8 mg/L 2IP plant growth regulators, then kept in a dark growth chamber for observation of their in vitro development. For the Plantform system, an immersion time of 3 hours was tested in a medium containing ½ MS for Cyclamen persicum. In the solid medium, embryo germination rates were found to be 39.82% for leaf explants and 50.77% for petiole explants. In the Plantform system, particularly for the CP5 genotype, when leaf embryos were used, a plant conversion success rate of up to 65% was achieved. Additionally, the average weight of embryos developed in the Plantform bioreactor system was recorded as 3.50 g, whereas in the solid medium, this value was 2.30 g. SPAD values were determined as 39.76 and 29.35, respectively. The results demonstrated that the Plantform bioreactor system provides an advantage over conventional solid culture in terms of plant conversion rate, embryo quality, and chlorophyll content. Keywords:Cylamen persicum ,micropropagation ,Plantform bioreactor system