Determining the essential oil composition of endemic Origanum acutidens (Hand.-Mazz.) ietswaart and antimicrobial activities

In this study the essential oil composition of the Origanum acutidens (Lamiaceae) plant, endemic species, and the antimicrobial activity were tested against the some selected bacteria and fungi species. The essential oil of the plant has obtained by using clevenger apparatus and the yield of the oil has determined as 1.5(v/w). The essential oil composition were analysed by using GC and GC – MS system quantittively and qualitatively. Carvacrol (32.11%), P – cymene (19.11%), Gamma terpinene (6.91%), Ethyl benzene (5.28%), Borneol (4.11%) components were found as major compounds in the Origanum acutidens essential oil. The antimicrobial activity of the essential oil of Origanum acutidens plant was tested against some selected bacteria Staphylococcus aureus ATCC25923, Bacillus megaterium DSM32; Escherichia coli ATCC25322, Klebsiella pneumoniae ATCC700603, Pseudomonas aeruginosa DMS50071, and some fungi and dermatophyte fungi strains, Candida albicans FMC17, Candida glabrata ATCC66032, Epidermophyton sp. and Trichophyton sp. by using Disc Diffusion and MIC (Minimal Inhibition Concentration) methods. In the antimicrobial activity of the Origanum acutidens essential oil, the oil has shown strong activity on the Staphylococcus aureus and middle activities on the Escherichia. coli, Bacillus megaterium from bacteria and Candida albicans and also Epidermophyton sp. Fungi. The results showed that the volatile oil of Origanum acutidens will be used as antibacterial and may be antifungal agent.