Investigation of protective effect of neoeriocitrin in INS-1E pancreatic beta cells in diabetic model

Diabetes is a very common metabolic disease and its incidence has been increasing. In addition to use of drugs in diabetes treatment, plant-based antidiabetic products are also used as alternative. Phytotherapeutic effects of various types of flavonoids such as anti￾inflammatory, antioxidant, antidiabetic, etc. are known. Pancreatic beta cell damage is an important factor in that leads to type 2 diabetes. In type-2 diabetes, the cause of the damage is not known exactly, it is asserted that it is mainly because of oxidative stress. Although antioxidant effects of flavonoids are well known, there is not much information enough about neoeriocitrin flavonoid. Neoeriocitrin is a flavonoid, which found in fruit extracts and fruit juices of some plants containing many Citrus species. In this study, antioxidant and antidiabetic effects of neoeriocitrin (0,25 µM, 0,5 µM ve 1 µM concentrations) were examined in INS-1E pancreas ß cell line by exposing to streptozotocin (STZ) in diabetic model. In this study, cell viability were examined by crystal violet, trypan blue and acridine orange/propidium iodide analyses; while oxidative stress were analysed by reactive oxygen species (ROS) and malondialdehyde (MDA) measurements. Also, the effect of neoeriocitrin to insulin response of INS-1E cells according to glucose level in media has been determined. N-acetyl L-cysteine (NAC), known as antidiabetic and antioxidant, has been used as a positive control. Cell viability analyses showed that neoeriocitrin increased the cell viability in a concentration-dependent manner. On the other hand, according to results of oxidative stress test, neoeriocitrin decreased the ROS and MDA levels depending on the concentration. Our insulin test results showed that neoeriocitrin caused increase in insülin response of the INS-1E cells according to the glucose amount. As a conclusion, our findings suggest that neoeriocitrin could protect oxidative damage in pancreatic beta cells and might be as a novel agent for diabetes. Keywords: Diabetic model, flavonoid, protective effect, neoeriocitrin, oxidative stress, INS-1E pancreatic beta cell line