Investigation of some viruses in tomato production areas of Diyarbakir province by molecular methods and molecular characterization of some virus isolates

Survey studies were conducted to determine the presence of Tomato yellow leaf curl virus(TYLCV), Potato virus Y (PVY), Tomato mosaic virus (ToMV), Tomato spotted wilt virus (TSWV) and Tomato ringspot virus (ToRSV) in tomato production areas of Diyarbakir province in the year 2018. PCR method was implemented to investigate the presence of TYLCV, Multiplex-RT-PCR method to PVY and ToMV infections, and RT-PCR method was implemented to investigate the presence of TSWV and ToRSV in tomato samples. A total of 278 leaf samples with and without virus symptoms were randomly collected in tomato production areas. It was determined that 56 (20.1%) of the samples tested by molecular methods were infected with at least one virus. Of the samples tested, 41 were infected with ToMV (14.7%), 21 were infected with PVY (7.6%) and 6 were contained mix infections with ToMV + PVY (2.2%). TYLCV, TSWV and ToRSV infections were not detected in the tomato samples. Four of PVY and four of ToMV isolates were randomly selected, coat protein genes were characterized, and their nucleotide sequences were recorded in GenBank. ToMV Diyarbakir isolates showed 99-100% similarity with other isolates in the world, while PVY isolates showed similarity between 88-99%. With this study, ToMV and PVY were reported for the first time in tomato production areas in Diyarbakir.