Nanobiotechnological manipulation of docetaxel to cancer cells

The compatibility of the chosen polymer with the drug used in cancer treatments determines the fate of the nanoparticle in the treatment. There are limited studies on the effect of resomer differences on the physical and chemical properties of nanoparticles, as well as on their biological activities. Therefore, in this study, it was aimed to formulate docetaxel-loaded polylactic-co-glycolic acid nanoparticles with different molecular weights (Resomer 502 and 504) and terminal groups (Resomer 502H and 504H) and to determine the nanoparticle characterization of these resomers and their effects on prostate cancer and healthy cells. Docetaxel loaded PLGA nanoparticles were prepared by single emulsion solvent evaporation method. Surface characterizations were performed with a zeta dimensioner and scanning electron microscopy. Encapsulation efficiency, in vitro drug release profiles and cytotoxic activities were determined. As a result of the study, it was determined that the main effect on the surface morphology of nanoparticles was by the molecular weight differentiation of the polymer. In addition, groups with acid terminal function were found to have higher encapsulation and reaction efficiency. In vitro release was observed for all formulations after 334 hours at pH 7.4 and 240 hours at pH 5.6. In addition, the selective cytotoxicity of high molecular weight groups was also found to be quite interesting. In conclusion, it has been determined that the studied resomers, especially the RG 504H group, has the potential to be used as a low-dose and high-efficiency long-release drug delivery system in the treatment of prostate cancer.