Determination of α-amylase, α-glucosidase, tyrosinase, acetylcholine esterase enzyme inhibitory properties and essential fatty acid content of the eber sarisi plant

In this study, the inhibitory properties of α-amylase, α-glucosidase, acetylcholine esterase and tyrosinase enzymes and essential fatty acid content of Eber Sarısı (Thermopsis turcica) endemic plant were determined. Inhibition activities of enzymes were determined by using α-amylase, Caraway-Somoygi iodide/potassium iodide method, α-glucosidase Palanisamy method, and acetylcholine esterase enzyme Ellman method and tyrosinase using L-DOPA dopachrome method. In determining the amount of essential fatty acid and its components, hydrodestilation process (HD) was applied with the use of Clevenger device and analyzed in GC-MS. Palmitic acid (51.8%), myristic acid (17.5%) and hexahydropharynyl acetone (5.3%) were the most abundant in plant fatty acid content. It has been determined that the species does not show inhibition activity against acetyl choline esterase and tyrosinase enzymes. Properties of the species that acetone and diethyl ether extract inhibit α-amylase The diethylether extract has been found to exhibit standard substance acarbose dial inhibition activity. It is seen that the methanol and acetone extracts of the plant have a high effect on inhibiting α-glucosidase enzyme. It is important with the studies on the antidiabetic effects of this valuable species, which is in danger of extinction.