Elucidation and characterization of glycosidic compounds from Cephalaria tuteliana (Dipsacaeae)

In this study, it is aimed isolation, purification, structural determination studies and to investigate biological activities of endemic Cephalaria tuteliana (Dipsacaeae). The plant material was collected during flowering period and dried under suitable conditions to be ready for research. The dried plant material was extracted with methanol and then separated into fractions by n-butanol:water extraction. The n-butanol phase, known to be rich in saponins, was extracted with n-hexane to remove apolar and oily parts. The chromatographic techniques such as vacuum liquid chromatography, medium pressure liquid chromatography, open column chromatography and thin layer chromatography were applied to butanol phase and 2 aglycones, 1 iridoid glycoside and 10 saponins, totally 13 compounds were obtained. The exact structures of the isolated compounds were determined using (1D- and 2D-) NMR. Among isolated compounds, two aglycones, (1-2) and a saponin (12) were obtained from Dipsaceaea family which include Cephalaria species, for the first time. The cytotoxic activities of two obtained aglycones were examined against different cell lines for discussing structure activity relationship.