İn vitro micropropagation of endemic plant Campanula tomentosa

İn this research it is aimed to determine establishment the most suitable nutrition media to get callus culture from seeds of Campanula tomentosa,that was critically endangered endemic plant belongs to Campanulaceae family which naturally grown in Turkey's West Anatolia region. In the first phase of study; Studies that searching effect of basic medias on germination, nutrition medias were different effects over seeds germination rate.Therefore compare the differrences between seeds germination rate on 4 basic nutrition medias seeds of Campanula tomentosa, used as a material.Sterile seeds were germinated based culture on MS ,1/2 MS , N6 , B5 medium free plant growth regulators (PGRs) .The highest germination rate was seen in ½ MS (%33success) media without plant growth regulator. In the second phase of study; İn tissue culture if study is about producing callus , using seeds to obtain explants from sterilized and healthy seedlings will be success to rejenerate. To produce callus ,explants transfer and cultured in MS, ½ MS , N6 and B5 medias with plant growth regulators in different concentrations (0.1 mg/l ve 3 mg/l)of 1α-Nafthalene aseticacid(NAA) that are used as a auxin and different concentrations (1 mg/l ve 4 mg/l) of benzylaminopurine (BAP) that are used as a cytokinin.Although more shoots were obtained in 1 mg/l BAP medium,growth of shoots were not good, callus structure was not compact and the colour of callus was yellowish or brown( 2nd type of callus) .In addition , 4 mg/l BAP of medium , growth of shoots were good , strong,compact and faded green-white colour(1st type of callus). In the third phase of study; effects of different nutrition medias on callus structure and colour and producing 1st type of callus and 2nd type of callus (%) In the fourth phase of study; Four different types of medium was prepared to investigate effects of NAA and BAP to proliferation processes. The best media for shoot development was MS.