Investigation of the impact of application of different plant growth regulators on Aronia (Aronia melanocarpa (michx) Elliot)micropropagation

The low rate of germination of Aronia melanocarpa species, whose value added has increased significantly in recent years, and the production of plants obtained by seed, which is a generative method, show expansion. Therefore, the usage of micro-propagation techniques has more importance for the production of Aronya species. This thesis has investigated the mass production of Aronya plant which will contribute to fruit diversity of our country with tissue culture methods which allow rapid, virus and disease-free proliferation. For that purpose, ' Viking '' and '' Nero nodal segments of Aronya are used as explants, and different doses of sodium hypochlorite (5%, 10%, 20%) in surface sterilization, different plant growth regulators, chemicals that will increase the success of shoot development, tillering and rooting and various combinations of regulators and chemicals are used to identify the best practices for mass production. The best result in surface sterilization is obtained from 10% sodium hypochlorite treated 10 minutes. DKW (Driver and Kuniyuki) containing Gamborg B5 Vitamins as basic plant media, MS (Murashige and Skoog), plant growth regulators (0,1mg/L from IBA and 0,1mg/L of GA3) and different doses of cytokinin source (BAP and KINETIN), 0.5, 1 ve 2 mg/L, are preferred. The best shoot growth and the maximum brooding are obtained from the combination of 2mg/L BAP+0.5 KİN + 0. 1mg/L l IBA+0.1mg/L GA3. ½ MS primary nutrient containing Gamborg B5 Vitamins, different doses of IBA (1, 2 ve 3 mg/L) and NAA' (1, 2 ve 3 mg/L) are used and best result is achieved from the plant media combined of 2 mg/l IBA +0,5 mg/l NAA. Key Words: Aronya, micro duplication, BBD, brooding, rooting