Production of pullulanase from Fontibacillus pullulanilyticus DSHK107T
2019
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Advisor: Prof. Dr. Hatice Güvenmez
Abstract (EN)
In this study, pullulanase was produced by a new type of genus of Fontibacillus; Fontibacillus pullulanilyticus DSHK107T isolated from soil of Cukurova University campus and the produced enzyme has been characterized. Total 110 strains were inoculated into producing medias (%1 starch and %1 pullulan contained) and incubated at 37°C for 72 hours. Then for the detection of pullulanase activity the bacteria culture was stained with iodine solution. 14 of them showed pullulanase activity. The partially purified pullulanase has optimum activity at pH 8.0, 30°C. According to these results due to the characteristics of the enzyme, it can be suggested that industrial field use is appropriate. Key Words: Fontibacillus pullulanilyticus DSHK107T, pullulanase, pullulan, enzyme production
Author
Dr. Damla Sihay
How to Cite
Damla Sihay (Master Thesis). Production of pullulanase from Fontibacillus pullulanilyticus DSHK107T, 2019, Çukurova University.
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