Genotyping of cytomegalovirus glycoprotein N and GB and its relationship with abortion status in an Iraqi female sample

In this study, 120 blood samples was collected from pregnant women attending different hospitals in Anbar. which were obtained during the period from December 2021 to June 2022, information was gathered about the pregnant women's ages (16-46) and residences (urban or rural). All samples have been tested for the presence of both IgG and IgM using Rapid Test, ELISA and PCR. The results in the current study showed Rapid test the existence of a high IgG positive (63.3%) rate and an IgM positive (33%) rate. The ELISA IgG test, the results of the examination showed that the highest positive percentage was in the age group 20-29 years (29.2%), while the lowest percentage (9.17%) was in the < 20 years group. The rural infection percentage (15.8%) was highest than the urban infection rate (3.33%). The results of testing the gN and gB genes by PCR showed that percentage (13.3%), (12.5%) were associated with abortions, (12.5%), (4.17%) in patients who did not have abortions, respectively. The results of the current study show a genotyping of isolates taken from pregnant women by comparing the category of aborted and non-abortive women, this heterogeneity may be related to cases of abortion in pregnant women