Neuroprotective effects of gentiana olivieri griseb

The genus Gentiana has 400 species in the world and 12 species in Türkiye, and plants belonging to this genus have had a wide range of uses in folk medicine for many years. Gentiana olivieri Griseb. used in Southeastern and Eastern Anatolia as an appetite stimulant, bitter tonic, and antidiabetic, as well as for the relief of stiffness and frostbite in terrified people. In this region, it is known as "Afat" or "Afat otu". In this study, which is a continuation of the studies carried out in our department, the aim was to determine the phytochemical content of the aerial parts of Gentiana olivieri obtained commercially from the Gaziantep region and to investigate their neuroprotective effects. For this purpose, decoction, ethanol, water, chloroform and n-butanol extracts of G. olivieri were prepared, and their neuroprotective effects were tested on the human neuroblastoma cell line SH-SY5Y. When the effects on intracellular cytokine levels induced by LPS were evaluated, ethanol and water extracts significantly increased IFN-α, aqueous extract IFN-β, chloroform and water extracts c1q, n-butanol and chloroform c3 and aqueous extract c9 levels. 1 secoiridoide glycoside (svertiamarin) and 1 oligosaccharide were purified from n-butanol extract by chromatographic studies. The structures of the isolated compounds were elucidated using various spectroscopic methods (13C NMR, 1H NMR, DEPT, and COSY techniques). In addition, the metabolites of svertiamarin, sveroside, gentiopicroside and isoorientin, which were previously obtained from Gentiana species, were detected in G. olivieri extracts by LC-qTOF-MS. Key words: Gentiana olivieri, secoiridoide, neuroprotective effect, isolation