Characterization of Helicobacter pylori, research of inhibition effects of honey and propolis samples on bacteria and urease

In this study, it was aimed to determine the antimicrobial and antibiotic activities of Helicobacter pylori, a bacterial effect of gastric ulcer in total 30 honey and propolis samples obtained from different sources in our country. H. pylori strains obtained from gastric biopsy specimens taken from patients with stomach ulcer complaints and standard HP J99 strains were produced under appropriate conditions. Sensitivities of HP1, 11 and 13 strains to four different antibiotics were tested by disk diffusion method. Only HP11 strains were found to be resistant to norfloxacin. All of the honey samples were found to have antimicrobial activity against H. pylori, while the strongest activity was observed in ivy (60 mm zone diameter) obtained from forties. HP9 was found to be a relatively resistant strain against honey samples. Strong anti-helicobacter activity was also detected in shrubs, oak, cow and hay. Overall, very high anti-helicobacter activity was detected in all of the propolis samples. The highest activity was observed in the sample (47 mm) obtained from Ereğli (P11), followed by P15, P12, P13, P14 and P6 samples respectively. The values obtained were measured as 2.67 to 18.12 mg / mL. The lowest value (2.67 ± 0.11) was determined in the vine (B10) obtained from Kırklareli. The IC50 value was measured <25.32 ± 0.23 according to the AA standard of all other honey samples. Strong anti-urease activity was detected in all of the propolis samples. The highest inhibition was observed in the samples from P11-15. In both honey and propolis samples, the highest antimicrobial and anti-urease activities were observed in the same specimens, indicating that the two separate tests were correct. It was concluded that honey and propolis samples may be an important alternative in the treatment of H. pylori infections.