Preventive effects of Hydroxytyrosol on liver damage due to aluminum exposure

The present study investigated the preventive effects of hydroxythyrosol, a powerful antioxidant compound which is found in pure olive oil, on liver damage caused by aluminum toxicity. Swiss albino mice were grouped into four (Group 1 - Control: 0.9% NaCl, Group 2 - AlCl 3: once 25mg / kg, Group III - HT: 10mg / kg / day-7 days, Group IV - AlCl3 + HT: 25mg / kg once and 10 mg / kg / day-7days respectively ) and studies were made based on their liver tissues. All applications were performed intraperitoneally (i.p.). Superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation (LP), which are indicators of oxidative stress (OS) level, were measured biochemically. Histological structure of the liver was examined under light microscope. Proliferating cell nuclear antigen (PCNA) were measured with immunofluorescence analysis to determine the changes in the cell cycle regeneration. Expression levels of the BRCA1and BRCA2 gene regions were measured by immunofluorescence analysis to determine DNA damage repair response. HT prevents Kupffer cell hyperplasia, necrosis, inflammation and decreases LP in the tissue. BRCA1, BRCA2 and PCNA findings revealed that HT induces DNA repair and supports normal process of the cell cycle. It is determined that protective effects of HT on cell survival and normal physiologic process is related to the significant support to endogenous antioxidant capacity. Keywords: Aluminum, Hydroxytyrosol, Liver, Oxidative stress, DNA damage repair response