Comparison of MDA , tas , TLR4 measurement and therapeutic response values in serum and tissue value in patients with inflammatory bowel disease.

Our study was performed with patients who had abdominal pain, bloody or bloodless diarrhea, weigt loss, nausea, vomiting in the Bülent Ecevit University Faculty of Medicine Education Research Hospital Gastroenterology Polyclinic. 44 Patients were detected at the ileocolonoscope 22 with ulcerative colitis and 22 with Crohn's disease. We identified our control group as 22 healthy adults. Our study was conducted prospectively. Patients previously treated for inflammatory bowel disease were not included in the our study. Blood and stool specimens were taken from the patients for diagnosis and the control. Truelove-Witts and Mayo clinical activity indeces were calculated in patients with ulcerative colitis. Crohn activity index calculated in Crohn's patients. Our patients were in the active period. Samples of inflamed mucosa and intact tissue were taken simultaneously with ileocolonoscopic for each patient. We aimed to show the sensitivity and specificity of fecal calprotectin test for diagnosed disease aktivite. Our second aim was to compare the disease activity of fecal calprotectin and other observed parameters. Finally, we checked for the determination value of activity that could take the place of invasive procedures. CBC, ESR, CRP, albumin. stool microscopy, serum, tissue, TAS, MDA, TLR4 was a measurement. Relations between these values were examined with respect to fecal calprotectin and disease activity. ESR and CRP changes were significant in Crohn's patients. This meaningful change in the UC was also added to albümin. Serum, tissue TLR4, TAS, MDA values were compared in our healthy control group and our patients. We found that tissue TLR4 and MDA changes were significant in the Crohn's patients. In our ulcerative colitis group, serum TLR4 and tissue MDA values were significantly changed. In addition, fecal calprotectin levels were correlated with disease activities and these biochemical values. There was a positive positive correlation between CDAI and fecal calprotectin in our Crohn's disease. (r=0,886 p=0,000). A strong positive correlation was found between ulcerative colitis in Mayo, Truelove Witts with fecal calprotectin. (Respectively; r=0,958 p=0,000/ r=0,944 p=0,000). There was no significant relationship in our patients between fecal calprotectin and TLR4, TAS, MDA. There was a statistically significant relationship between fecal calprotectin and CRP, ESR in both our Crohn's disease and ulcerative colitis patients. In our study, the level of fecal calprotectin was highest in the presence of active disease findings, fecal calprotectin level measured in all patients remitted after treatment was found to be 50-100 μg / g. (p=0,001). In the gastrointestinal tract In general, values above 200 ug / g for fecal calprotectin indicate the presence of an inflammatory active organic disease. In our study, the distinctive cut-off value of fecal calprotectin was 260 μg / g for active or remnant IBD. For this value was AUC (area under the curve): 0,850, 83-87 % CI, p=0,001. In summary; When we evaluate our results in our study. Summary; we thought that determination in of disease activity and even in the follow-up of disease might be used to monitored of levels of fecal calprotectin for ulcerative colitis and Crohn disease.