The detection of tospoviruses in ornamental plants in hobby gardens and landscaping areas in Adalar district of Istanbul province

This study was conducted in order to detect Tomato Spotted Wilt Virus (TSWV), Irish Yellow Spot Virus (IYSV) and Impatients Necrotik Spot Virus (INSV) in ornamental plants growing hobby gardens and landscaping areas in Adalar district (Büyükada, Heybeliada, Kınalıada and Burgazada) of Istanbul provınce between 2015 and 2016. During the surveys carried out in the field, the samples were collected from simptomatologically suspicious ornamental plants and the ornamental plants which did not show symptoms. All of the collected samples (150) were firstly tested by ELISA method and the samples were not found to be infected with TSWV and INSV. The samples were detected to be infected with IYSV by ELISA and they were used in RT-PCR studies. At the end of the RT-PCR studies using the IYSV-465c;IYSV-239f primer pair, a band with a size of 240 bp was observed in the agarose gel electrophoresis (1.5%) and bunlardan Pittosporum tobira ve Hydrangea macrophylla were found to be infected with IYSV molecularly.