Analysis of volatile components and trace elements in clove plant (Syzygium aromaticum)

Extraction with clevenger system and microwave method were used to obtain volatile components of clove (Syzygium aromaticum) which is a medical and aromatic plant. GC - MS analyzes were performed using three different libraries. FTIR library search was carried out to obtain information about the structure of organic substances in the solid state clove. For each component obtained as a result of GC-MS and FTIR analysis; health, food, cosmetic and pharmaceutical applications and their beneficial and toxic effects were examined. The results obtained from GC-MS were compared and interpreted with drug active ingredients. In the determination of trace elements, powdered cloves were carbon coated and subjected to macro and micro element screening in SEM-EDX as a preliminary study to provide information about its composition and morphology. The elements and compounds of the clove were examined by XRF. Quantitative analysis of Fe, Zn, Cu, Mn, Cr, Mo, Co, Cd, K, Ca, Mg, Na was performed on ICP - MS device. The components obtained as a result of analysis with GC – MS device are alpha farnesene, methyl-5-hepten-2-one, 2-caryophylene oxide, 2-nonanone, isoeugenol, linalool, beta-caryophylene, alpha-cube, benzaldehyde, ethylbenzoate, 9- octadecanoic acid is benzylalcohol, hepta-oxa-cyclohene, furfural, benzylbenzoate, furoxane, methyl salicylate, naphthalene, valensene, hexanoic acid and delta-3-carene. Quantitative analysis of the clove of Madagascar, with the ICP-MS device, calcium (5951 µg / Kg), magnesium (1801.2 µg / Kg), sodium (1447.8 µg / Kg), potassium (1659.6 µg / Kg) , chrome (113,13 µg / Kg), iron (1,743 µg / Kg), manganese (46,381 µg / Kg), cobalt (14,6 µg / Kg), copper (629.8 µg / Kg), zinc (334) , 4 µg / Kg), molybdenum (22.17 µg / Kg) and cadmium (14.79 µg / Kg). In cloves of Indonesia, calcium (5287.7 µg / Kg), magnesium (2108.2 µg / Kg), sodium (1432.2 µg / Kg), potassium (1622.8 µg / Kg), chromium (132.21 µg / Kg), iron (1,408 µg / Kg), manganese (43.52 µg / Kg), cobalt (16.83 µg / Kg), copper (581.8 µg / Kg), zinc (352.4 µg / Kg) , molybdenum (16.98 µg / Kg) and cadmium (15.41 µg / Kg). With the FTIR device, eugenol, anhydrous quinine, 1,2,4-trimethylbenzene, p-anisidine and ethanol components were detected. KEYWORDS : Clove, volatile component, GC-MS, ICP-MS, trace element, AES, IR, SEM-EDX