Nanoplatforms attached schiff bases by condensation method; investigation of glucose oxidase enzyme as biocatalysts

Polymer-bound Schiff-base ligands were prepared from Polystyrene A-NH2 (PSA) and salicylaldehyde (Sal) and 5-methylsalicylaldehyde (5-SalCH3). Firstly, all of the modified polymers were characterized on the basis of FT-IR and GPC spectral analyses studies. The changes in the surface structure of modified polymers were examined with SEM-EDX analysis. After that, Glucose oxsidase were immobilized onto (PSA), (PSA?SalH) and (PSA-SalCH3) modified polymer with using the adsorption method. The influence of temperature, pH and reusability, storage capacity on the free and immobilized Glucos oxsidase were investigated. The immobilized glucose oxidase on (PSA) showed two optimum pH values that were pH = 4 and pH = 7 (PSA-Sal)-GOx and (PSA-SalCH3)-GOx were showed optimum pH values as 7 and 6, respectively. (PSA) and (PSA-SalH) were showed optimum temperature as 80 oC. Kinetic parameters were calculated as 1.641mM min?1/ 1.031 mM.; 1.266 mM min?1 / 0.917 mM, 1.069 mM min-1 / 0.549 mM and 2.216 mM min?1 / 0.813 mM, for Km /Vmax respectively; (PSA), (PSA-SalH) and (PSA-SalCH3) bound enzymes.