Evaluation of Tbx3 protein level during differentiation of hematopoietic stem cells obtained from cord blood to myeloid series

Hematopoiesis, is all the cells within the blood system being formed in a specific hierarchy from a small community of hematopoietic stem cells (HSC). In this process, the HSC community primarily forms progenitor cells, later these cells continue to differentiate and specialise to form completely differentiated mature blood cells. Shortly, hematopoiesis appears when the events of cell division and differentiation occur sequentially. HSCs generating healthy blood cells is subject to the physiological control mechanisms working properly. One of these control mechanisms is the "transcriptional control" mechanism which enables suitable genes to be identified and regulated at the right time, with an adequate amount and in a sufficient time. The disappearance or damage of physiological control in the process of hematopoiesis, results in the uncontrolled proliferation of cells and specialised cells return to their unspecialised form which then causes cancer to be formed. In previous studies performed by ourteam, the peripheral blood and bone marrow samples of patients with different types of haematological cancer were shown to have high levels of Tbx3 named transcription factor. The Tbx3 protein has been found to be increased in not only haematological cancer but many cancer types. These findings show that Tbx3 could play a role in cell differentiation. The aim of this study was to identify at which stages of physiological development the Tbx3 protein was found in hematopoiesis. With this purpose, from the umbilical cord blood (the source of hematopoietic stem cells), HSCs were obtained and in a cultured environment differentiation to myeloid series was ensured. The presence of Tbx3 protein was for the first time observed and shown in the hematopoeitik stem cells obtained from umbilical cord blood and myeloid series cells. The level of HSCs in the Tbx3 protein and the myeloid series was compared. No statistical significance in terms of the level of the Tbx3 protein was observed between the two.