Radioactive labeling of L-proline-graft-poly (HEMA) nanopolymer and in vivo evaluation on experimental animals

The use of nanotechnology in today's world opens new hopes in the diognasis and treatment of various diseases. Therefore, importance of nanotechnology has increased day after day. Especially, nanotechnology in cancer treatment and diognasis has an imprortant potential. Nanotechnology provides significant contributions to diagnosis, treatment, medicine, artificial tissue informationand technology The specific advantages of the radiolabelled nanopolymers and the variety of areas of use have been an indispensable instrument for us in the field of health. It is essential that these polymers are biologically similar and adjustable in the design of radiolabelled polymers. Radiolabeled nanopolymers provide maximum therapeutic effect with minimal side effects designed for target cell and tissue. It is very important that the radiopharmaceutical used in the treatment and diagnosis of cancer is inserted into the target cell. For this purpose, it will be possible to carry the previously synthesized L-proline-graft-poly (HEMA) nanopolymer with technetium-99m (99mTc) radionuclide. This labeled polymer will be a new radiopharmaceutical used in the field of Nuclear Medicine. In this study, firstly, L-proline-graft-poly (HEMA), a new generation polymer previously synthesized, was labeled with (99mTc) by determining appropriate conditions. Thin Layer Radio Chromotography (TLRC) and High Perfromance Liquid Chromatography (HPLC) method were used to optimize labeling studies. Secondly, L-proline-graft-poly (HEMA) In vivo study labeled with 99mTc has been performed on experimental animals. In vivo stage, scintigraphy and biodistribution studies were performed on Albino Wistar rats. As a result of scintigraphy and biodistribution studies, it has been determined which organs are affected by L-prolin-graft-poly (HEMA) nanopolymer specifically. In this study, for the first time, in vivo studies of 99mTc labeled L-proline-graft-poly (HEMA) nanopolymer were performed and the first preliminary studies were carried out for imaging and treatment of this polymer in the field of Nuclear Medicine.