Investigation of the effects of lactic acid bacterial filtrates on obesity and cholesterol by in vitro methods

Obesity is a disease caused by an imbalance between energy intake and energy expenditure, which leads to excessive amounts of fat and it is also a risk factor associated with the emergence of various diseases such as coronary heart disease, hypertension, type 2 diabetes, cancer, respiratory complications, and osteoarthritis. Cholesterol is a sterol compound that is taken with animal-derived nutrients and is found in the stomach and bile ducts. Although it is an essential element for body tissues, elevated cholesterol levels are an important risk factor for coronary heart disease. Recently there has been increased interest in LAB's, especially for lactobacilli, due to antidiabetic, anti-pathogenic, anti-cancer effects, as well as lowering of serum cholesterol levels and stimulation of the immune system. Based on the information, 3T3-L1 (preadipocyte) cells used for investigating the effects of selected lactic acid bacteria to adipogenesis and obesity. For this purpose, experimental steps followed are bacterial population growth, control of purity and storage, testing of cholesterol removal of bacteria and filtrates, determination of Gamma-Aminobutyric Acid production of bacteria, cultivation and differentiation of 3T3-L1 cells, in vitro cytotoxic action determination studies (MTT Measurement), Oil-Red-O dyeing (Microscopic Detection by Oil Cells staining). As a result, cholesterol-lowering and adipogenesis repressing effects of selected bacteria on 3T3-L1 cell line were determined.