Lenfoma kanseri ve kanser metastazının erken teşhisi için biyosensör üretimi ve uygulaması

Lymphoma cancer ranked as the fifth cancer type is seen all around the world and death caused by lymphoma shows increment day after day. To prevention of death caused by lymphoma cancer, in the conducted study we fabricated rGO-FET biosensor device for early detection of lymphoma cancer. To do so, we synthesized the reduced graphene oxide from bulk graphite as sensing area. The fabrication of FET base structure was done by using microelectronic technology and rGO was transferred onto FET structure for use as sensing area. The characterization of rGO was completed by investigation XRD, Raman, FTIR and TEM analysis. These analysis results showed that synthesized rGO is suitable for detection. The first trials of rGO-FET biosensor were completed with various pH solutions. The pH trials revealed that the capability of rGO-FET biosensor device. For the early detection of lymphoma, the miRNA sequences were used for functionalization. The probe sequence was immobilized onto rGO sensing area for the miRNA studies. miRNA-155 sequence was dropped onto probe sequence and electrical measurements has been completed by analyzing I-V curves. The I-V curves revealed that the probe sequence immobilized on rGO matched with various concentration contained miRNA-155 sequence samples and shift was observed towards to left side with increasing concentration. The specificity study revealed that the probe sequence only matched with miRNA-155 sequence. The selectivity studies showed that rGO-FET biosensor can distinguish various concentrations. The results showed that rGO-FET can define miRNA-155 concentrations between 100 pM and 10 µM. The Limit of detection (LOD) was determined as 100 pM. Overall results suggested that the rGO-FET biosensor device successfully fabricated and can be used for early detection of lymphoma cancer and cancer metastasis.