Molecular detection of asymptomatic bacteruria among pregnant women with anemia in Iraq

The purpose of the research is detection of the relationship between anemia in pregnant women in the second trimester of pregnancy and virulence factors for bacterial infections of Escherichia. coli (E. coli) by PCR. 150 midstream urine samples were collected from pregnant women with age (16-45) years, also were taken blood samples. The percentage of bacterial growth which was isolated from 105 samples, 44,3 % (n=47) were E. coli followed by other gram negative bacteria, 11,3% (n=12) which include Klebsiella pneumonia, and 10,4% (n=11) Pseudomonas aeruginosa. Also was the number and percentage 50% (n=35) of the infection group women had anemia, while 100% (n=35) of the non-infected women and non-anemic. Further confirmation done using by API 20E system and VITEK 2. Purity and concentration of the extracted DNA from 47 isolates were confirmed with Nano-drop. The concentration ranged from 50-200 mg/µL. This study proved, the appearance of cnf1 gene in the E. coli bacteria which is infect to pregnant women with anemia, also showed the toxic role of these genes in tissue damage.