Irak gayara ilçesinde insanlarda kutanöz leıshmanıasisin moleküler tespiti

This study aimed to determine the prevalence of parasites among Iraqi citizens in the study area in Iraq by using classical and molecular methods to obtain information about the degree of parasitism in the population. This study included 2148 cases of CL among the people of Nineveh governorate, Al-Qayarra during the study period, of which 451 were positive and 1697 were negative cases after being diagnosed by dermatologists in Al-Qayara subdistrict hospital. The microscopic diagnosis was used for the direct detection of the parasitic infection for 451 positive samples. 62 samples out of 451 samples (as an example of 451 samples) were chosen for the culture diagnosis. Of these 62 samples, 21 (33.9 %) had parasite growth at the promastigote stage and appeared in the culture medium after 21 days, while 14 (22.6%) did not show any growth. The other samples had contamination and were 27 (43.5%). It was chosen ten samples out of 21 samples (as an example of 21 samples) that had parasite growth to detect the target gene Cytochrome b (Cytb) was used to identify CL by a molecular study using QPCR. The results showed that all of the duplicates for 9 samples were positive, while 1 of the duplicate (sample number 9) was positive which might indicate a low amount of parasitic DNA, it appeared the sensitivity of real-time PCR was 90%.