Development of a new electrochemical biosensors systemfor simultaneous detection of NADH and H2O2

The aim of the study is to develop a new electrochemical biosensor system for the simultaneous determination of nicotinamide adenine dinucleotide (NADH) and hydrogen peroxide (H2O2). SPCE/RGO/PNR/AuNP and SPCDE/RGO/PNR electrodes are prepared modifying reduced graphene oxide (RGO), polyneutral red (PNR), gold nanoparticle (AuNP), screen-printed carbon electrodes (SPCE) and dual-screen-printed carbon electrodes (SPCDE) electrodes. These electrodes are electrochemically characterized by cyclic voltammetry. These developed systems are used for the determination of NADH, H2O2 and simultaneous determination of NADH and H2O2. In the first part of the study, the sensitivity of SPCE/RGO/PNR/AuNP electrodes to NADH and H2O2 is primarily tested and their use as individually NADH and H2O2 sensors is examined. In these analyses, performance criteria such as optimum operating conditions (pH, working potential), storage and operation stability and repeatability are determination for detection of NADH and H2O2. In the second part of the study, simultaneous amperometric detection of NADH and H2O2 with SPCDE/RGO/PNR/AuNP electrodes is determined under optimum operating conditions specified in the first part. Analytical characterization of the electrochemical biosensor system developed for the simultaneous determination of NADH and H2O2 is carried out. Currently developed NADH, H2O2 and dual NADH and H2O2 biosensors are used in artificial blood serum samples, for the determination of NADH and H2O2 and the simultaneous determination of NADH and H2O2 and successful results are obtained.