Examination of effectiveness on Yersinia ruckeri that is bacterial fish pathogen of ozone application

In this study, the susceptibility of Yersinia ruckeri to ozone gas was investigated. Y. ruckeri (ATCC 29473) inoculated on tryptic strain agar (TSA) medium and was incubated for 24-48 hours at 22 ° C. At the end of the incubation period, the concentration of 1.5x108 cfu / ml bacterial suspension according to 0.5 Mcfarland's (OD600nm = 0.132 absorbance by UV spectrophotometer) of the pure culture obtained were prepared in tubes containing 100 μl of physiological saline (FTS - 0,9% NaCl - Sigma). The following to serial dilutions (1:10 rate), the bacterial concentration (1.5x104 cfu/ml) was exposed to ozone gas at different times (5, 10, 15 and 30 min). The susceptibility of Y. ruckeri to ozone gas was determined by helping generator at 12 and 40 γ/ml concentrations. Obtained the data indicated that the most effective bactericidal effect was determined in 5 and 10 min by 12 γ / ml ozone application of Y. ruckeri. However, in the following time, it was observed that ozone lost its activity. At the same time, bactericidal activity increased depending on time in the ozone application of 40 γ/ml and all bacteria dead within 15 and 30 minutes. At the end of this trial, it was determined by the bactericidal effect that Y. ruckeri was sensitive to ozone gas.