Detection of metal ions and oxidant agents with pillar[5]arene-based fluorescence sensors, imaging in live cells and brain tissue of zebra fish

In this thesis study, the effective detection of metal ions and oxidant agents that can accumulate in body cells was investigated through fluorescence-based macrocycles. For this purpose, various intermediate products and two target fluorescence Pillar[5]arene derivatives were synthesized using multiple synthesis steps and different purification techniques. Bodipy compound derivatives were preferred as the source of fluorescence character and were chemically bonded to the Pillar[5]arene main bodies by a "click reaction". Here, the structures were elucidated using various characterization methods such as NMR, FT-IR, mass spectroscopy. The obtained target macrocycles were investigated by spectrophotometric methods for the detection of metal ions and oxidant ions. UV-vis and fluorescence spectrophotometers were used for absorption and emission graphs and values, and approximately a total of over a thousand samples were analyzed for this thesis study. The prepared target images were given special short names due to their macrocyclic structure, and the target images were named Bodipy-P[5]aren and 5B-P[5]aren, respectively. As a result of spectroscopic measurements, it was concluded that the fluoride macrocycle called Bodipy-P[5]arene is sensitive to MnO4- and Hg2+ ions and can be used as a sensor for these analytes along with other analytical measurements through selective processes. Similarly, the fluorescent macrocycle presented as 5B-P[5]arene showed sensitive and selective properties towards Cr2O72- and Hg2+ ions, and it was concluded that it can be used as a sensor for these analytes along with other analytical measurements. Imaging studies of fluorescence macrocycles, which were determined to be used as sensors for targeted analysis as a result of spectroscopic data, were also carried out on live cells and zebrafish brain tissue. The results obtained with spectrophotometric devices are taken live and are compatible with the results on zebrafish brain tissue.