PCOS model developed in rats showing the effects of treatment angiotensin II receptor blocker losartan with histological and biochemical parameters

Average 8-12 weeks old Sprague-Dawley rats (n = 21); At standard conditions (21-22 C, 55-65% in a humid environment, 12 h 12 h light-dark cycle) were fed rat chow pellets and water ready to drink was released (ad libitum). cases that share the same nutritional conditions were included in the study. 14 PCOS rat model was created, 7 rats were enrolled as normal control and have not implemented any action. Then all the rats under anesthesia and blood samples for parameters to be studied and underwent bilateral oophorectomy, serum was collected for all rats. After the rats were sacrificed by cervical dislocation. Histologic sections of follicular structure oophorectomy materials (antral follicle count) were evaluated changes. AMH in blood samples using ELISA technique (anti-mullerian hormone), IGF-1 (Insulin-like growth factor) and lipid peroxidation indicator were measured as malondialdehyde levels. Histologically, PCOS model developed and saline placebo (0.9% NaCl), field rats, primary ovarian tissue compared to the normal group, and decreased the number of secondary follicles, increased the number of tertiary follicles. PCOS field of losartan in rats compared to saline group at primary ovarian tissue and an increased number of secondary follicles, the number of tertiary follicles decreased significantly. PCOS model developed and saline placebo (0.9% NaCl) field in rats it was significantly higher than the normal group the AMH. PCOS model developed and saline placebo (0.9% NaCl) field in rats it was significantly higher than the normal group the AMH. Losartan treatment PCOS field rats (p = 6.62 ± 1.53) than in the placebo saline group AMH levels were significantly decreased (p = 8.11 ± 0.56). PCOS model developed and saline placebo (0.9% NaCl), field rats, the IGF has increased significantly compared to the normal group (p = 4.63 ± 0.44). Losartan in the PCOS group (p = 4.97 ± 0.66), placebo, there was no significant difference between the group receiving saline (Table 2). PCOS model developed and saline placebo (0.9% NaCl) (0.19 ± 0.045) field rats, normal group (0.17 ± 0.022) compared markers of oxidative stress in plasma malondialdehyde (MDA) has not changed. In the PCOS grunen losartan (p = 0.18 ± 0.045), saline placebo group (p = 0.19 ± 0.045) were not significant differences between the amount of plasma MDA.