Preparati̇on of molecularly i̇mpri̇nted column materi̇al for propranolol preconcentrati̇on

Propranolol, have been used for treating chest pain (angina) for many years and still frequently used drug with β-receptor blockers effect. The major effect of propanol was found on the cardiovascular system and bronchi. Propranolol was considered as antianginal, antiarrhythmic, and antihypertensive agent. It has been frequently used a spectrophotometric method to determine the vitro amount. Furthermore, other tekniks like as radyoimmunoassay, gas chromatography (GC) and high-performance liquid chromatography (HPLC) were used. The present study determined a low amounts of propranolol for preparing a new solid-phase extraction system. For this purpose, we have synthesized highly selective molecular imprinted polymer and which further was embedded into the cryogen colon. In preparing a mulecular imprinted polymer, molecular from propranolol, afunctional monomer, methacrylic acid, crosslinker EGDMA, acetonitrile solvent, and AIBN as insitiator were used. The cryogen was selected because of the following advantages like large pores, short diffusion path, and pressure reduction. For preparation, the crosslinking MBAAm, functional monomer HEMA and as an initiator APS, TEMED were used. Obtained molecularly imprinted polymer and embedded MIP cryogel structure, have been identified and using scanned electron microscope (SEM), fourier transformed infrared spectroscopy (FTIR). Prepared colon material adsorption and the desorption terms were optimized. MIP has embedded highest adsorption capacity for cryogel 200 ppm propranolol when used 106.24 mg/g, respectively. Embedded propranolol MIP connected with cryogel that found Kd value 769.23 M and Qmax 723.61 mg/g. Prepared a solid-phase extraction colon as well as the test batch system, for similar propranolol selection, beta- blockers and active ingredient a dideral once again was used as the medicament preconcentration. All analysis have done with HPLC device. Propranolol has achieved 89.59% rate of recovery. The results for determination of propranolol preconcentration or selective chromatographic separation were prepared by using cheap and long-lasting new solid-phase extraction material.