First time determination of prevalence of the two amphibian pathogen(Batrachochytri̇um dendrobati̇di̇s ve Ranavi̇rus) around Eği̇rdi̇r Lake using real-time PCR

In this study, two amphibian pathogens were identified on endemic Pelophylax caralitanus which is located in Eğirdir Lake in Isparta and listed as Near Threatened (NT) on Red List by IUCN and also known as to be decreasing, using the Real – Time PCR (quantitative PCR, qPCR) technique. That two amphibian pahtogens are Batrachochytrium dendrobatidis and Ranavirus (Frog virüs, FV-3). In Eğirdir Lake, that pathogen organisms was determined for the first time using qPCR technique with specific primers. And also we aimed grow B. dendrobatidis in the medium in laboratuar conditions. P. caralitanus samples were collected with swab technique for twice between 2012 and 2013 years from 6 localities in field. Results of experiment of 2012 yaer were %17,02 positive for Batrachochytrium dendrobatidis and totaly negative for Ranavirus. On the other hands, results of 2013 were %45 positive for Batrachochytrium dendrobatidis and %5 positive for Ranavirus. Furthermore, we have examined B. dendrobatidis cultures under light microscope and we have detected sporangia, mature and immature sporahifas, zoosporangiums and hapolid zoospores. Consequently, that two deadly pathogens which responsible deadly disases and decreasing on amphibian populations; determinations of Batrachochytrium dendrobatidis (Bd) and Ranavirus (Iridoviridae) have been srudied and first records of that pathogens in Eğirdir Lake have determined.