Expression analysis of MDR subfamily from ABC proteins in berries of sultana seedless grape variety

The ABC Proteins / ATP Binding Cassette Transporters / ABC Transporters protein family is one of the largest and most diverse protein families during the evolution. ABC proteins consist of proteins that are involved in the exchange of substances with the internal and external environment of the cell, are localized on the membranes of cells and organelles. These proteins include proteins involved in many metabolic pathways such as regulation of chromosome structure, protein secretion, drug resistance, antibody formation, phytohormone transport and signal transduction. In this study, the expression profile of the putative ABCB / MDR gene family of the Vitis vinifera, whose genome project has been completed was analyzed during the different stages of development in grapes berries. Expression profile of the 19 genes belonging to Vitis ABCB / MDR sub-family, were obtained by real-time PCR at different periods of development in Sultaniye çekirdeksiz (Vitis vinifera) berries. The expression of VvMDR3, VvMDR12, VvMDR13, VvMDR14, VvMDR16 and VvMDR17 genes increased 4-6 weeks after flowering and decreased towards veraison. Expression of VvMDR7, VvMDR10, VvMDR11 and VvMDR15 genes was high at flowering, fruit set and after veraison, but decreased at a minimum level during ripening and over-ripening stage. Expression of VvMDR1, VvMDR2, and VvMDR8 genes was high at fruit set and before fruit set. The high expression of VvMDR genes during flowering and fruit set suggests that they may play a role in auxin transport during this period.