The isolation, production and investigate usage possibilities of aerob and anaerob bacteria enzymes that can help to hen digestive system

ABSTRACTMSc THESISTHE ISOLATION, PRODUCTION AND INVESTIGATE USAGEPOSSIBILITIES OF AEROB AND ANAEROB BACTERIA ENZYMESTHAT CAN HELP TO HEN DIGESTIVE SYSTEMÇUKUROVA UNIVERSITYINSTITUTE OF NATURAL AND APPLIED SCIENCESDEPARTMENT OF BIOLOGYSupervisor : Prof. Dr. Ömer ÇOLAKYear: 2006, Page:56Jury : Prof. Dr. Ömer ÇOLAKProf. Dr. BurhanARIKANAssoc. Prof.Dr. Gökhan CORALAssoc.Prof.Dr. Hatice GÜVENMEZAssoc.Prof.Dr. Nisa ÜNALDI CORALIn this study we aimed that, after isolation of Bacillus and Clostridium bacterias thatfounds naturally at seeds flora and received that being adapted the compounds of seeds,providing enzyme synthesis again intensive producting at seed based cultures.For this aim amylase, sellulase and lipase aktivities of Bacillus and Clostridiumwere detected which isolated from hen seeds. Enzymes wre producted with partialpurification from the strains that showed hihest enzyme activity analysed by theSDS-PAGE. According to this profile of each amylases B22, B23, B27, B28, B31, B41strains exist of two bands that molecular weights are chances in range of 62000-54000Daand 54000-47000Da. These enzymes activities were detected and animal experimentsperformed by productind great deal of enzyme.Consequently at first experiment as from second week the weight of experimentgroup chatched and pass the controls. However at second experiment from the first week thegroup of experiment 1 showed 20-50 grams weght increase also the experiment 2 groupshowed 30-40 grams for each week more than control group.Key Words: Amilase, Selülase, Lipase, SDS-PAGEII