Isolation of thermophilic amylase and cellulase (multienzymes) producing Bacillus sp. strains, enzyme production, characterization and investigation of biotechnological applications

In this study, the production and characterization of thermoalkaliphilic amylase and cellulase enzymes (multi enzymes) from Bacillus sp. strain which is isolated from soil samples were carried out. The bacterial strains containing the mentioned enzymes were isolated then, the best of selected and determined as Bacillus sp. SHK-7. After optimization of enzyme production conditions for temperature and pH, the enzymes were particular purified and characterized.The molecular weight of the cellulase enzyme was estimated to be 68.9 kDa as a single active band, by zymogram SDS-PAGE analyses. The maximum activity of the cellulase was at 60ºC and pH 8.0. The enzyme was stable in the presence various detergent. Maltose and maltose-derived oligosaccharide were determined as hydrolysis products by thin layer chromatography.The zymogram native PAGE analyses of the amylase enzyme revealed a two active band with molecular weight of 302 kDa and 453 kDa. The optimum temperature and pH of the amylase enzyme determined as 80ºC and pH 8.0. The enzyme was stable in the presence of various detergent and metal ions. Maltose and maltose-derived oligosaccharide were determined as hydrolysis products by thin layer chromatography.According to these results, SHK-7 cellulase and amylase enzymes are thermophile, alkaliphile, halotolerant, thermostable and alkali-stable. Owing to these characteristics, the cellulase and amylase enzymes obtained in this research can be used as additive in detergent, textile, food and drink industries.