Investigation the possible effects of tocopherol and dexpanthenol separately and together in an experimental colitis model induced by tnbs

Inflammatory Bowel Disease (IBD) is a systemic chronic disease that mainly affects the gastrointestinal tract, the etiology of which is unknown. In the pathogenesis of IBD, oxidative stress and inflammation are important risk factors. Dexpanthenol (Provitamin B5) is an alcohol form of pantothenic acid (Vitamin B5), which has anti-inflammatory and anti-irritant properties and stimulates wound healing. E Vitamin (tocopherol) is a fat-soluble essential vitamin that functions as an antioxidant in human body. In our study, effects of dexpanthenol and vitamin E on the Trinitrobenzene Sulfonic Acid (TNBS) colitis model in rats were investigated separately. In the study, 40 male Wistar-albino male rats (300-400 gr) were used. Rats were divided five equal groups; Sham control (was administered saline %0.009), TNBS, TNBS + tocopherol (T), TNBS + dexpanthenol (D), TNBS+ tocopherol + dexpanthenol (TD). TNBS group rats underwent anesthesia and were given TNBS with a rectal cannula. T group was given rectal injection of TNBS for 4 days followed by intraperitoneal injection of 30 IU/kg of T for 4 days, D group was given 500 mg/kg D for 4 days, TD group was given 30 IU/kg of T and 500 mg/kg of D intraperitoneally for 4 days . All of the rats were sacrificed at the end of 4 days and 10 cm columns were removed. The longitudinally divided colon segments were biochemically and histopathologically examined. Tissue MDA levels were significantly decreased in the D and TD treatment groups, MPO levels were significantly decreased in the T, D and TD treatment groups, GSH levels were significantly increased in the T, D and TD treatment groups and CAT levels did not significantly differ between the control, colitis and treatment groups. The antioxidant and anti-inflammatory properties of dexpanthenol and vitamin E have the potential to reduce TNBS-induced colitis tissue damage