Efficacy of entomopathogenic nematode isolates from Turkey and Kyrgyzstan against pest Sitophilus [S. granarius, S. oryzae, S. zeamais (Coleoptera: Curculionidae)] adults

İn this study Turkey and Kyrgyzstan belonging isolates of EPN which was two isolates of Heterorhabditis bacteriophora (81KG and TOK20) and two isolates of Steinernema feltiae (3KG and Tokat-Emir) were tested their activity against cereal important pest insect of Sitophilus genus [(Sitophilus granarius, Sitophilus oryzae and Sitophilus zeamais) (Coleoptera: Curculionidae)]. Experiments containing single dose were conducted in two times at 25±1ºC laboratory condition with five replications. Single dose applications were tested at concertrations of 50 infective larva (IJ) insect-1, daily controls were made and insect mortality rates (%) were subjected to statistical analysis. As a result the highest effect were observed on the two isolates S. feltiae (3KG and Tokat-Emir). İn dose-death applications, 5, 10, 50, 100, 250, 500 and 1000IJs insect-1 doses were applied against the adult of S. granarius, S. oryzae and S. zeamais insect. İn the result of this study, the isolates of S. feltiae 3KG and Tokat-Emir were shown their highest effect against Sitophilus genus at the concentration of 1000 IJ insect-1. An experiment containing the concentration with the highest effect on dose-death applications and different temperatures were conducted. As a result of the experiment carried out at 3 different temperatures (10, 15 and 20ºC), the most effective isolates against S. granarius were at 10ºC and at a dose of 1000 IJs in S. feltiae 3KG isolate (%85.00), and %100 at 15ºC and 20ºC and at a dose of 1000 IJs in same isolate. İn addition the most effective isolate against S.oryzae was S. feltiae 3KG isolate (%100) at a dose of 1000 IJs at 15ºC and 20ºC. Where as the most effective isolates against S. zeamias were S. feltiae Tokat-Emir and 3KG isolates (%100) at a dose of 1000 IJs at 15ºC and 20ºC.