Usage of organoboron based polymeric materials at enzymatic biosensors

The application and characterization of organoboron polymers to electrochemical enzymatic biosensors, which have only been tested in a limited number, have been examined within this thesis's scope, and patent applications will be made since this is one of the pioneering studies. Therefore, contribution to the literature will be made. In this thesis, the use of biocompatible organoboron polymers as enzyme immobilization molecule is added value to the biosensor system to be prepared in many ways. Utilizing the direct electropolymerization (one-step) method, an electrochemical enzymatic biosensor system was developed with polyaniline film coated carbon screen printed electrodes, and poly 3-aminophenylboronic acid film coated gold screen printed electrodes/ glassy carbon electrode, and novel organoboron polymer film-coated platinum screen printed electrodes/glassy carbon electrode. Organoboron polymer-based enzymatic and electrochemical analysis that will be developed in this thesis will be used for the determination of catechol that is one of the mostly analyzed phenolic compounds in the chemistry and agriculture industry. Catechol analysis was developed in which the tyrosinase enzyme was used as the sample determination system. With the developed biosensor system, the phenolic components were tested in the linear range between 1 µM to 200 µM with different electrodes. After the biosensor performance conditions optimization, real sample analysis were also performed for controlled catechol added green tea samples with 3% to 10% range of standard deviation results. Finally, it should be noted that the developed biosensor system can be designed and commercialized as a portable end product that allows real-time detection. Phenolic compounds, which are determined to determine the antioxidant and antimicrobial activities of natural foods, are partly made within the scope of quality control analysis, and the developed organoboron polymer-based biosensor system will allow faster, cheaper, precise, and real-time tests.