The effect of 3',4'-Dihydroxyflavonol on endoplasmic reticulum stress in the cerebellum during brain ischemia-reperfusion in rats

Ischemia is a condition that occurs as a result of insufficient blood flow to the tissues and negatively affects cellular energy production. Brain ischemia is a critical pathological process that causes neuronal function to deteriorate and cell death due to temporary or permanent interruption of cerebral blood circulation. During this process, triggering endoplasmic reticulum (ER) stress disrupts intracellular protein folding mechanisms, leading to increased neuronal damage. In the current study, the effect of 1-week 3',4'-Dihydroxyflavonol (DiOHF) supplementation on endoplasmic reticulum stress in cerebellum tissue after cerebral ischemia-reperfusion in rats was investigated. The study was conducted on male Wistar-albino rats. A total of 28 rats were used in the study and the groups were formed as follows. Control group (n=6): No anesthesia or surgical procedure was applied to the animals in this group. Sham group (n=6): After general anesthesia was induced in the animals in this group, the carotid artery regions were opened and closed. After the application, solvent application was performed for 1 week (1 ml DiOHF solvent). IschemiaReperfusion Group (n=8): After isolating the carotid arteries in rats under general anesthesia, ischemia was performed by ligating them for 30 minutes, followed by reperfusion. IschemiaReperfusion + DiOHF Group (n=8): After ischemia was performed by ligating the carotid arteries in rats under general anesthesia for 30 minutes, reperfusion was allowed. DiOHF supplementation was performed for 1 week. After one week of treatment, animals were killed under general anesthesia and cerebellum tissues were taken to evaluate GRP78, HSP70, CHOP, Bcl-2 and Bax levels. While I/R increased GRP78, HSP70, CHOP and Bax values in the cerebellum, it caused significant suppression in BCl-2 levels. However, 3',4'-Dihydroxyflavonol application for 1 week corrected the deteriorations caused by I/R. The study results show that 1 week of 3',4'-Dihydroxyflavonol treatment after I/R provides significant correction in endoplasmic reticulum stress related parameters caused by focal brain I/R in the cerebellum. Keywords: Ischemia/Reperfusion; 3',4'-Dihydroxyflavonol; GRP78, HSP70, CHOP, Bcl-2, Bax and rat