The comparison of angiogenic factors and expression of sirtuin gene in brown and white adipose tissues of rats treated with adrenomedullin and resveratrol in obesity

Obesity is a complex, chronic disease which arises according to the interaction between genetic and environmental factors. Many angiogenic factors provide formation of new vessels for the nourishment of tissues as in adipose tissue. Adrenomedullin (AdM) is also a peptide that has angiogenic features. Resveratrol is a molecule which is effective in the activation of sirtuin 1 (SIRT1) gene. SIRT1 gene has a regulatory effect on energy metabolism. The effects of resveratrol over SIRT1 gene expression, the variations of vascular endothelial growth factor (VEGF) due to their importance in development of brown and white adipose tissue, and effects of AdM were investigated. Rats were divided into 8 groups. Obese groups were fed with high fat diet which has 60% fat content as energy for 3 months. After providing obesity, 2.5 nmol/kg AdM and 10 mg/kg resveratrol were treated to experimet groups intraperitonally (i.p.) every other day for 4 weeks. AdM, SIRT1 and VEGF mRNA levels in brown and white adipose tissues were detected with semi-quantitative PCR; protein levels were detected with Western Blotting. White and brown adipose tissue groups have responsed differently to the treatments. Furthermore, obese and control groups have resposed differently to AdM and resveratrol treatments. SIRT1 expression has increased with resveratrol in white adipose tissue. AdM has no effect alone over the increase of expression, but the the expression has increased when treated with resveratrol. The treatment of AdM with resveratrol has revealed completely diverse responses than the treatment of these two molecules apart. The results have showed that AdM could have ability to increase SIRT1 protein levels, and resveratrol would have a role in angiogenesis.