The synthesis of aminoacid based amides and evaluation of anticancer effect on hela cervical cancer cells this compounds

In this study, lysine, phenylalanine, histidine, valine and proline amino acids were reacted with p-nitrobenzoyl chloride to give amide derivative compounds. The structure of these compounds was elucidated by FT-IR. The cytotoxic, apoptotic and angiogenic effects of these synthesized compounds were studied in vitro on the HeLa cervical cancer cell line. The cytotoxic activities of 25, 50, 100 and 200 μM doses of the synthesized compounds on HeLa cells were determined spectrophotometrically using the WST-I method. B1, B2 and B5 showed cytotoxic effect on HeLa cells whereas B3 and B4 compounds induced proliferation. To determine the apoptotic effect of the compounds on HeLa cells, the amount of poly (ADP-Ribose) Polymerase (PARP) molecule, which is the degradation product of caspases and termed the death molecule, was measured spectrophotometrically by ELISA method. The amount of vascular endothelial growth factor (VEGF) was also measured by elisa method to determine the angiogenic effect.