Biosorption, preconcentration, and determination of cd (II) ion, using amberlite xad-16 resin modified with anoxybacillus caldiproteolyticus and geobacillus stearothermophilus through faas

In this study isolation, identification and characterization of the bacteria obtained from soil sample of Ergani Makam Mountain was performed. Based on this study it was identified that was of thermophilic Anoxybacillus caldiproteolyticus. These bacteria whose characterization was performed and Geobacillus stearothermophilus obtained readily were immobilized on Amberlite XAD-16 and two different sorbents were acquired to be used for sorption studies. The resulting sorbents were loaded into separate mini columns. Cd (II) ions found in the solution were enriched using mini columns containing biosorbent and were determined by flame atomic absorption spectrometric method. The effects of parameters such as the pH of the solution on the separation and preconcentration efficiency, flow rate, eluent type, concentration and the sample volume were investigated. For Amberlite XAD-16 immobilized separately with A. caldiproteolyticus and G. stearothermophilus the recovery yields were determined as 98.23 ± 2.40 and 98.93 ± 1.3 (N = 5) under the experimentally optimum operating conditions and the Cd (II) ions retained in the column were recovered respectively through 1 M 10 ml of HCl and 0.5 M 2 ml of HNO3 solutions. For both biosorbents optimum pH= 6, and the flow rate was 2 ml / min in the enrichment studies. In order to investigate the applicability of the developed enrichment methods, the maximum recovery of matrix ions such as Na+, K+, Cl- and Na+ were examined. The accuracy of the proposed methods was analysed through standard reference material (SCP SCIENCE EnviroMAT Waste Water, Low (EU-L-2)) and the results obtained were supplemented by the methods we have developed. Methods were applied for the determination of Cd (II) ions in real samples such as Tigris River, the Hazar Lake and Diyarbakir city drinking water. Keywords: Anoxybacillus caldiproteolyticus, Geobacillus stearothermophilus, Biosorption, Amberlit XAD-16, FAAS